16s ribosomal dna rdna sequencing  (Thermo Fisher)

Journal: mSystems

Article Title: Spatially divergent metabolic impact of experimental toxoplasmosis: immunological and microbial correlates

doi: 10.1128/msystems.01126-25

Figure Lengend Snippet: Impact of acute and chronic T. gondii infection on the microbiome. ( A and B ) Beta diversity of bacterial components was compared using the Bray’s NMDS analysis of 16S rRNA gene sequencing at 15 days post-infection ( A ) or 50 days post-infection ( B ). Statistical significance was determined by Permutational multivariate analysis of variance (PERMANOVA). ( A ) Small intestine (Pr(>F) = 0.001, R 2 = 0.5984), cecum (Pr(>F) = 0.004, R 2 = 0.5727), and large intestine contents (Pr(>F) = 0.002, R 2 = 0.5616). ( B ) Small intestine (Pr(>F) = 0.001, R 2 = 0.4824), cecum (Pr(>F) = 0.006, R 2 = 0.5009), and large intestine contents (Pr(>F) = 0.001, R 2 = 0.5246). ( C–H ) At 15 days post-infection, the relative abundance of ( C ) Peptostreptococcaceae (median difference = 5.283, 95%CI = 1.387 to 13.28), ( D ) Clostridiaceae (median difference = 1.136, 95%CI = 0.324 to 4.005), ( E ) Bifidobacteriaceae (median difference = −3.015, 95%CI = −6.769 to 0.7419), ( F ) Erysipelotrichaceae (median difference = 12.76, 95%CI = −0.5948 to 29.81), ( G ) Muribaculaceae (median difference = −25.94, 95%CI = −52.160 to −2.312), and ( H ) Bacteroidaceae (median difference = 2.069, 95%CI = 0.069 to 3.994), in the small intestine (SI), cecum (CE), and large intestine (LI) contents from uninfected and infected mice. ( I–L ) At 50 days post-infection, the relative abundance of ( I ) Bifidobacteriaceae (median difference = 2.069, 95%CI = 0.069 to 3.994) , ( J ) Erysipelotrichaceae (median difference = −0.546, 95%CI = −0.116 to −0.012), ( K ) Desulfovibrionaceae (median difference = 0.287, 95%CI = −0.4785 to 1.156), and ( L ) Muribaculaceae (median difference = 0.089, 95%CI = 0.038 to 0.168) in the intestinal contents from uninfected and infected mice. Error bars represent Welch’s unpaired t -test ± SEM and Hodges-Lehmann median differences. 15 day uninfected N = 10, 15 day infected N = 9, 50 day uninfected N = 10, 50 day infected N = 7.

Article Snippet: 16S ribosomal DNA gene sequencing was performed by Novogene.

Techniques: Infection, Sequencing

Journal: Frontiers in Endocrinology

Article Title: Sex differences in the skeletal muscle response to metformin treatment and the possible association with gut-muscle axis in rats

doi: 10.3389/fendo.2025.1650805

Figure Lengend Snippet: Effects of metformin treatment on gut microbiota composition in male and female rats. (A, B) Chao1 index and Shannon index for male and female rats; (C) PCoA 3D plot of weighted UniFrac distances in 16S rRNA sequencing of colonic contents in control (CT) male and female rats; (D, E) PCoA 3D plot of 16S rRNA sequencing of colonic contents in CT and metformin (MT) treated male and female rats; (F, G) Relative abundance of gut microbiota at genus levels in colonic contents of male and female rats. Each dot represents data from one rat; Male: CT, n = 7; MT, n = 7; Female: CT, n = 6; MT, n = 6. * P < 0.05, MT vs. CT.

Article Snippet: The fecal specimens were sent to GENEWIZ, Inc. (Suzhou, China) for 16S ribosomal DNA gene sequencing.

Techniques: Sequencing, Control